Abstract
Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) is used in determining the genetic relationships based upon PCR and restriction analysis. Specific genes, such as small subunit ribosomal RNA gene (16S rDNA), large subunit ribosomal RNA gene (23S rDNA), 16S-23S rRNA intergenic spacer (IGS) and symbiotic genes, have been used in PCR-RFLP. The PCR-RFLP profile is used to estimate the genetic diversity of microorganisms. The PCR-RFLP method has been used successfully in the differentiation of rhizobial species.
Keywords: Amplified 16S rDNA restriction analysis (ARDRA), Dendrogram, Endonuclease, Genotypic diversity, Large subunit ribosomal RNA gene (23S rDNA), Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Rhizobia, Small subunit ribosomal RNA gene (16S rDNA), Symbiotic gene, 16S-23S rRNA intergenic spacer (IGS).
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