Abstract
Two-dimensional electrophoresis has, for many years, been the primary workhorse for performing functional proteomics, the large-scale analysis of protein expression differences. Despite its merits, limitations inherent to this technology have been recognized for a long time, ranging from its gel-to-gel variability to its inability to represent several classes of proteins. Recently, however, technical advances in two-dimensional electrophoresis have alleviated several of these drawbacks. Fractionation approaches prior to two-dimensional electrophoresis, e.g. by chromatography, organelle fractionation or Equalizer Beads technology, have increased the number of visible proteins. Fluorescent two-dimensional difference gel electrophoresis has boosted the quantitative aspects of two-dimensional electrophoresis. New protein stains have also enabled the analysis of post-translationally modified proteins. As a result, two-dimensional electrophoresis has been thoroughly modernized, enabling it to remain the preferred method for protein expression analysis in a large number of laboratories. In this review we will give an overview of these technological advances.
Keywords: Two-dimensionalelectrophoresis, Proteomics, Phosphorylation, Glycosylation, 2-D DIGE, Protein prefractionation
Current Analytical Chemistry
Title: High Resolution Protein Display by Two-Dimensional Electrophoresis
Volume: 5 Issue: 2
Author(s): Gert Van den Bergh and Lutgarde Arckens
Affiliation:
Keywords: Two-dimensionalelectrophoresis, Proteomics, Phosphorylation, Glycosylation, 2-D DIGE, Protein prefractionation
Abstract: Two-dimensional electrophoresis has, for many years, been the primary workhorse for performing functional proteomics, the large-scale analysis of protein expression differences. Despite its merits, limitations inherent to this technology have been recognized for a long time, ranging from its gel-to-gel variability to its inability to represent several classes of proteins. Recently, however, technical advances in two-dimensional electrophoresis have alleviated several of these drawbacks. Fractionation approaches prior to two-dimensional electrophoresis, e.g. by chromatography, organelle fractionation or Equalizer Beads technology, have increased the number of visible proteins. Fluorescent two-dimensional difference gel electrophoresis has boosted the quantitative aspects of two-dimensional electrophoresis. New protein stains have also enabled the analysis of post-translationally modified proteins. As a result, two-dimensional electrophoresis has been thoroughly modernized, enabling it to remain the preferred method for protein expression analysis in a large number of laboratories. In this review we will give an overview of these technological advances.
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Cite this article as:
Bergh Van den Gert and Arckens Lutgarde, High Resolution Protein Display by Two-Dimensional Electrophoresis, Current Analytical Chemistry 2009; 5 (2) . https://dx.doi.org/10.2174/157341109787846199
DOI https://dx.doi.org/10.2174/157341109787846199 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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