An HPLC-MS/MS Method for the Quantitative Determination of Ticagrelor and its Active Metabolite AR-C124910XX in Human Plasma and its Application to a Clinical Study

Title:An HPLC-MS/MS Method for the Quantitative Determination of Ticagrelor and its Active Metabolite AR-C124910XX in Human Plasma and its Application to a Clinical Study

Volume: 13 Issue: 3

Author(s): Jingbo Peng, Yicheng Wang, Mupeng Li, Chenjie He, Yao Chen, Xiaoping Chen, Dongsheng Ouyang, Yuanfei Huang and Zhirong Tan*

Affiliation:

• Department of Clinical Pharmacology, XiangYa Hospital, Central South University, 410078, Changsha, Hunan,China

Keywords: Ticagrelor, AR-C124910XX, HPLC-MS/MS, method of validation, pharmacokinetics, Chinese male volunteers.

Abstract: Background: Ticagrelor is a reversibly binding oral P2Y12 receptor antagonist used clinically for the prevention of thrombotic events. A selective, rapid, and reliable method based on highperformance liquid chromatography–tandem mass spectrometry was developed and validated for the quantification of ticagrelor and its active metabolite AR-C124910XX in human plasma.

Method: After liquid–liquid extraction with methyl tert-butyl ether, ticagrelor and its metabolite ARC124910XX were chromatographed on a C18 column with a mobile phase consisting of acetonitrile– water containing 10 mM ammonium acetate (60:40, volume [v]/v) at a flow rate of 0.30 mL/min. Detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring and with a positive electrospray ionization interface using [M+H]+ ions with the following transitions: m/z 522.9 → m/z 152.9 for ticagrelor, m/z 478.9 → m/z 152.9 for AR-C124910XX, and m/z 530.2 → m/z 152.9 for the internal standard.

Results: Calibration curves were established in the range 4.019–2017 ng/mL for ticagrelor and 2.889– 1040 ng/mL for AR-C124910XX. The lower limits of quantification for ticagrelor and AR-C124910XX were determined to be 4.019 and 2.889 ng/mL, respectively.

Conclusion: The method was fully validated and successfully applied to determine ticagrelor and ARC124910XX simultaneously in human plasma and proved to be suitable for pharmacokinetic study of ticagrelor in healthy Chinese male volunteers.

Cite this article as:

Peng Jingbo, Wang Yicheng, Li Mupeng, He Chenjie, Chen Yao, Chen Xiaoping, Ouyang Dongsheng, Huang Yuanfei and Tan Zhirong*, An HPLC-MS/MS Method for the Quantitative Determination of Ticagrelor and its Active Metabolite AR-C124910XX in Human Plasma and its Application to a Clinical Study, Current Pharmaceutical Analysis 2017; 13 (3) . https://dx.doi.org/10.2174/1573412912666160518151458

DOI https://dx.doi.org/10.2174/1573412912666160518151458	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X