Abstract
A new HPLC method have been developed and validated for simultaneous determination of carprofen, vitamin B1, B6 and B12 in veterinary pharmaceutical preparations medicines. The method used a Hypersil GOLD aQ (250 x 4.6 mm, 5 µm) column, under the following chromatographic conditions: gradient elution, using a mobile phase composed by phosphate buffer (pH 2.65) and methanol as an organic modifier. The mobile phase was delivered at a flow rate of 1 mL/min and the detection was made by photodiode array (PDA) detector at 292 nm. This method was fully validated and applied on available pharmaceutical product. The limit of detection (LOD) and limit of quantitation (LOQ) for vitamin B1 were 0.5ng/mL and 1.5ng/mL respectively while for vitamin B12 were 1ng/mL and 5ng/mL respectively The main advantages of this HPLC method relies on the possibility to asses in a single run all the above mentioned drugs.
Keywords: Carprofen, vitamin B1, B6 and B12, HPLC, pharmaceutical analysis
Current Analytical Chemistry
Title:Determination of Carprofen in the Presence of some Hydrosoluble Vitamins of Group B by HPLC Method
Volume: 11 Issue: 4
Author(s): Georgeta-Simona Stan, Bianca Elena Mitoi, Andrei Mihaila, Irinel Adriana Badea and Hassan Y. Aboul-Enein
Affiliation:
Keywords: Carprofen, vitamin B1, B6 and B12, HPLC, pharmaceutical analysis
Abstract: A new HPLC method have been developed and validated for simultaneous determination of carprofen, vitamin B1, B6 and B12 in veterinary pharmaceutical preparations medicines. The method used a Hypersil GOLD aQ (250 x 4.6 mm, 5 µm) column, under the following chromatographic conditions: gradient elution, using a mobile phase composed by phosphate buffer (pH 2.65) and methanol as an organic modifier. The mobile phase was delivered at a flow rate of 1 mL/min and the detection was made by photodiode array (PDA) detector at 292 nm. This method was fully validated and applied on available pharmaceutical product. The limit of detection (LOD) and limit of quantitation (LOQ) for vitamin B1 were 0.5ng/mL and 1.5ng/mL respectively while for vitamin B12 were 1ng/mL and 5ng/mL respectively The main advantages of this HPLC method relies on the possibility to asses in a single run all the above mentioned drugs.
Export Options
About this article
Cite this article as:
Stan Georgeta-Simona, Mitoi Elena Bianca, Mihaila Andrei, Badea Adriana Irinel and Aboul-Enein Y. Hassan, Determination of Carprofen in the Presence of some Hydrosoluble Vitamins of Group B by HPLC Method, Current Analytical Chemistry 2015; 11 (4) . https://dx.doi.org/10.2174/1573411011666150326001707
DOI https://dx.doi.org/10.2174/1573411011666150326001707 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Chemokines as Pharmacological Targets
Mini-Reviews in Medicinal Chemistry The Diabetic Charcot Foot
Current Diabetes Reviews Melanogenesis and Melanosome Transportation Modulators from Medicinal Plants
Letters in Drug Design & Discovery The Effects of Physical and Psychological Stress on the Gastrointestinal Tract: Lessons from Animal Models
Current Molecular Medicine Assessment of Key Elements in the Innate Immunity System Among Patients with HIV, HCV, and Coinfections of HIV/HCV
Current HIV Research Editorial from Guest Editor. Chronic Cough
Current Respiratory Medicine Reviews New Agents Promote Neuroprotection in Parkinson’s Disease Models
CNS & Neurological Disorders - Drug Targets Radiation-Induced Stress Proteins - the Role of Heat Shock Proteins (HSP) in Anti- Tumor Responses
Current Medicinal Chemistry Dendritic Cell Homeostasis in the Regulation of Self-Reactivity
Current Pharmaceutical Design New Adamantane Derivatives with Sigma Affinity and Antiproliferative Activity
Medicinal Chemistry Therapeutic Drugs that Behave as Mechanism-Based Inhibitors of Cytochrome P450 3A4
Current Drug Metabolism Multiple Myeloma and the Immune Microenvironment
Current Cancer Drug Targets Patent on Biomarkers in Medical Research: A Focus on Neuropsychiatric Disorders
Recent Patents on Biomarkers Gastrin: An Acid-Releasing, Proliferative and Immunomodulatory Peptide?
Mini-Reviews in Medicinal Chemistry Clinical Value of Measuring the Renin/Aldosterone Levels: Optimising the Management of Uncontrolled/Resistant Hypertension
Current Vascular Pharmacology Selective Sensitization to Penicillin V with Tolerance to Other Betalactams
Recent Patents on Inflammation & Allergy Drug Discovery Therapeutic Potential of Cholesteryl O-acyl α-glucoside Found in Helicobacter pylori
Current Medicinal Chemistry Vascular Risk Factors and Lesions of Vascular Nature in Magnetic Resonance as Predictors of Progression to Dementia in Patients with Mild Cognitive Impairment
Current Alzheimer Research Development of Multifunctional Nanoparticles for Targeted Drug Delivery and Noninvasive Imaging of Therapeutic Effect
Current Drug Discovery Technologies Beyond Promoter: The Role of Macrophage in Invasion and Progression of Renal Cell Carcinoma
Current Stem Cell Research & Therapy