Abstract
A fundamental problem in cancer research is identification of the cells responsible for tumor formation. The latest field of cancer research has revealed the existence and role of cancer stem cells (CSCs). These findings support the idea that malignancies originate from a small fraction of cancer cells that show self-renewal and multi- or pluripotency. Identification of this CSC population has important implications for the management of cancer patients, including diagnostic and predictive laboratory assays as well as novel therapeutic strategies that specifically target CSCs. In this study, we investigated the growth rates of CSC populations for comparison with cancer cell lines. To construct the growth curves, blood-derived CSCs were isolated from patients with breast, colon, or lung cancer and cultured in vitro. Quantitative real-time PCR was then performed to identify CSCs in the samples. We found that CSCs did not follow the common pattern of a typical growth curve of mammalian cells in contrast to the cancer cell lines. This observation of rapidly growing CSCs indicates their involvement in tumor formation.
Keywords: Cancer stem cells, growth curves, Nanog, Oct3/4, Sox2.
Current Stem Cell Research & Therapy
Title:Comparison of the Growth Curves of Cancer Cells and Cancer Stem Cells
Volume: 9 Issue: 2
Author(s): Maria Toloudi, Eleni Ioannou, Marina Chatziioannou, Panagiotis Apostolou, Christos Kiritsis, Stella Manta, Dimitrios Komiotis and Ioannis Papasotiriou
Affiliation:
Keywords: Cancer stem cells, growth curves, Nanog, Oct3/4, Sox2.
Abstract: A fundamental problem in cancer research is identification of the cells responsible for tumor formation. The latest field of cancer research has revealed the existence and role of cancer stem cells (CSCs). These findings support the idea that malignancies originate from a small fraction of cancer cells that show self-renewal and multi- or pluripotency. Identification of this CSC population has important implications for the management of cancer patients, including diagnostic and predictive laboratory assays as well as novel therapeutic strategies that specifically target CSCs. In this study, we investigated the growth rates of CSC populations for comparison with cancer cell lines. To construct the growth curves, blood-derived CSCs were isolated from patients with breast, colon, or lung cancer and cultured in vitro. Quantitative real-time PCR was then performed to identify CSCs in the samples. We found that CSCs did not follow the common pattern of a typical growth curve of mammalian cells in contrast to the cancer cell lines. This observation of rapidly growing CSCs indicates their involvement in tumor formation.
Export Options
About this article
Cite this article as:
Toloudi Maria, Ioannou Eleni, Chatziioannou Marina, Apostolou Panagiotis, Kiritsis Christos, Manta Stella, Komiotis Dimitrios and Papasotiriou Ioannis, Comparison of the Growth Curves of Cancer Cells and Cancer Stem Cells, Current Stem Cell Research & Therapy 2014; 9 (2) . https://dx.doi.org/10.2174/1574888X0902140121163539
DOI https://dx.doi.org/10.2174/1574888X0902140121163539 |
Print ISSN 1574-888X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3946 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Design, Synthesis and Biological Evaluation of Novel N-hydroxyheptanamides Incorporating 6-hydroxy-2-methylquinazolin-4(3H)-ones as Histone Deacetylase Inhibitors and Cytotoxic Agents
Anti-Cancer Agents in Medicinal Chemistry Base Excision Repair: Contribution to Tumorigenesis and Target in Anticancer Treatment Paradigms
Current Medicinal Chemistry Synthesis, Radiosynthesis and Metabolism of 131I-Y-c(CGRRAGGSC)
Anti-Cancer Agents in Medicinal Chemistry An Overview of Current Applications of Nanotechnology in Biomedical Research: A Patent Survey
Recent Patents on Nanomedicine Peptide Vaccines for Cancer Therapy
Recent Patents on Inflammation & Allergy Drug Discovery Synergistic Interactions between GW8510 and Gemcitabine in an In Vitro Model of Pancreatic Cancer
Anti-Cancer Agents in Medicinal Chemistry The Protein-Protein Interaction-Mediated Inactivation of PTEN
Current Molecular Medicine Chemoprevention Strategies for High Risk Women
Current Women`s Health Reviews Diketoacid Inhibitors of HIV-1 Integrase: From L-708,906 to Raltegravir and Beyond
Current Medicinal Chemistry Drug-Induced Hypokalaemia
Current Drug Safety Cdc42 Signaling Pathway Inhibition as a Therapeutic Target in Ras- Related Cancers
Current Medicinal Chemistry The Cross-over of Anticancer Agents with Osteoclast Activities
Current Cancer Therapy Reviews Meet Our Editor-in-Chief
Clinical Cancer Drugs Reprogrammed Metabolism of Cancer Cells as a Potential Therapeutic Target
Current Pharmaceutical Design Bisphosphonate Anticancer Activity in Prostate Cancer and Other Genitourinary Cancers
Anti-Cancer Agents in Medicinal Chemistry Modulation of Immuno-biome during Radio-sensitization of Tumors by Glycolytic Inhibitors
Current Medicinal Chemistry Role of Lycopene in the Control of ROS-Mediated Cell Growth: Implications in Cancer Prevention
Current Medicinal Chemistry Himalayan Plants as a Source of Anti-Cancer Agents: A Review
The Natural Products Journal Antitumor Effect of Cycle Inhibiting Factor Expression in Colon Cancer <i>via Salmonella</i> VNP20009
Anti-Cancer Agents in Medicinal Chemistry Differential Regulation of CYP3A4 and CYP3A5 and its Implication in Drug Discovery
Current Drug Metabolism